Comet Assay Kit (3-well slides) from Abcam

Product Specs
ItemComet Assay Kit (3-well slides)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab238544
Quantity15 TEST, 75 TEST
ApplicationsCellular Assay
TargetDNA damage

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Abcam

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United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Adaptive changes in the DNA damage response during skeletal muscle cell differentiationFrontiers in Cell and Developmental BiologyNovember 27, 2023Inês Faleiro, Ana I. Afonso, André Balsinha, Beatriz Lucas, Robert M. Martin, Edgar R. Gomes, Sérgio F. de Almeidacommercially available comet assay kit from Abcam and followed the protocol provided within the kit (ab238544). Images were acquired using the LSM 980 point scanning confocal microscopes using a 20× objective.

(2) Anticancer Effects of 6-Gingerol through Downregulating Iron Transport and PD-L1 Expression in Non-Small Cell Lung Cancer CellsCellsNovember 15, 2023Dong Young Kang, Sanghyeon Park, Kyoung Seob Song, Se Won Bae, Jeong-Sang Lee, Kyoung-Jin Jang, Yeong-Min Parkmanufacturer’s protocol, essentially as described previously [1]. 2.8. Comet Assay A comet assay kit (Abcam; #ab238544) was used to measure cellular DNA damage, essentially as described previously [1]. 2.9. Apoptosis Analysis The

(3) A CRISPR-drug perturbational map for identifying compounds to combine with commonly used chemotherapeuticsNature CommunicationsNovember 13, 2023Hyeong-Min Lee, William C. Wright, Min Pan, Jonathan Low, Duane Currier, Jie Fang, Shivendra Singh, Stephanie Nance, Ian Delahunty, Yuna Kim, et al.for 72 h. Comet assay Comet assay was performed according to the manufacturer’s protocol (Abcam, ab238544). Vehicle or drug treated BE2C and GIMEN cells were harvested and resuspended in cold 1x PBS at 100

(4) Non-small cell lung cancers (NSCLCs) oncolysis using coxsackievirus B5 and synergistic DNA-damage response inhibitorsSignal Transduction and Targeted TherapySeptember 25, 2023Bopei Cui, Lifang Song, Qian Wang, Kelei Li, Qian He, Xing Wu, Fan Gao, Mingchen Liu, Chaoqiang An, Qiushuang Gao, et al.double-strand breaks were measured and quantified by single-cell gel electrophoresis using the CometAssay Kit (ab238544, Abcam). Comets were analyzed using the CaspLab software to obtain the average tail moment (TM). At

(5) ATF3 induction prevents precocious activation of skeletal muscle stem cell by regulating H2B expressionNat CommunAugust 17, 2023Suyang Zhang, Feng Yang, Yile Huang, Liangqiang He, Yuying Li, Yi Ching Esther Wan, Yingzhe Ding, Kui Ming Chan, Ting Xie, Hao Sun, et al.dosages and the comet assay was operated immediately after irradiation through a Comet Assay Kit (Abcam, ab238544) according to the manufacturer’s instructions. The amount of DNA damage was indicated by the tail moment

(6) MAGI1 Prevents Senescence and Promotes the DNA Damage Response in ER+ Breast CancerCellsJuly 25, 2023Janine Wörthmüller, Simona Disler, Sylvain Pradervand, François Richard, Lisa Haerri, Gustavo A. Ruiz Buendía, Nadine Fournier, Christine Desmedt, Curzio Rüeggas described above and analyzed for DNA fragmentation after 48 h using a comet assay kit (Cat. No. ab238544, Abcam). Briefly, the comet slides were coated with 35 μL of agarose to form a base layer; then, 75

(7) Polo‑like kinase 1 selective inhibitor BI2536 (dihydropteridinone) disrupts centrosome homeostasis via ATM‑ERK cascade in adrenocortical carcinomaOncology ReportsJuly 20, 2023Ruei-Ci Lin, Yu-Ying Chao, Wei-Chih Lien, Huei-Cih Chang, Shih-Wei Tsai, Chia-Yih Wangobservation. Comet assay The comet assay was performed according to the manufacturer's instructions (cat. no. ab238544; Abcam). Briefly, the comet agarose (1%) was loaded onto the comet slide to form a base layer. Then,

(8) PARP1 Inhibition Halts EBV+ Lymphoma Progression by Disrupting the EBNA2/MYC AxisbioRxivJuly 12, 2023Giorgia Napoletani, Samantha S. Soldan, Toshitha Kannan, Sarah Preston-Alp, Peter Vogel, Davide Maestri, Lisa Beatrice Caruso, Andrew Kossenkov, Asher Sobotka, Paul M. Lieberman, et al.performed on DMSO, BMN-673 and Etoposide treated cells using the Comet Assay Kit protocol (Abcam; Cat. No. ab238544), with minor optimizations. Specifically, LCL were lysate overnight at 4°C, and the electrophoresis

(9) Streptozotocin induces renal proximal tubular injury through p53 signaling activationScientific reportsMay 29, 2023Nakai K, Umehara M, Minamida A, Yamauchi-Sawada H, Sunahara Y, Matoba Y, Okuno-Ozeki N, Nakamura I, Nakata T, Yagi-Tomita A, et al.Health, Bethesda, MD). Comet assay The comet assay was performed using the Comet Assay Kit (Abcam ab238544) as previously described41,44. In brief, mouse kidneys were removed and minced in a small amount of

(10) Synergism of FAK and ROS1 inhibitors in the treatment of CDH1-deficient cancers mediated by FAK-YAP signalingInternational Journal of Biological SciencesMay 15, 2023Jiaming Gao, Yunying Yao, Chenxuan Liu, Xi Xie, Donghe Li, Ping Liu, Zaiqi Wang, Baoyuan Zhang, Ruibao Renanalyzed by flow cytometry. Comet assay The assay was performed using the Comet Assay Kit from Abcam (ab238544) as described by the manufacturer. Briefly, cells in RPMI medium were mixed with comet agarose 1:10

(11) Nuclear DJ-1 Regulates DNA Damage Repair via the Regulation of PARP1 ActivityInternational journal of molecular sciencesMay 12, 2023Wang ZX, Liu Y, Li YL, Wei Q, Lin RR, Kang R, Ruan Y, Lin ZH, Xue NJ, Zhang BR, et al.primer sequences are shown in Supplementary Table S1. 4.11. Comet Assay The comet assay kit (Abcam, ab238544, UK) was used to perform the comet assay. Cells were first mixed with agarose and then laid on comet

(12) Combination of natural polyphenols with a precursor of NAD+ and a TLR2/6 ligand lipopeptide protects mice against lethal γ radiationJournal of Advanced ResearchMarch 1, 2023Elena Obrador, Rosario Salvador-Palmer, Blanca Pellicer, Rafael López-Blanch, J. Antoni Sirerol, Juan I. Villaescusa, Alegría Montoro, Ryan W. Dellinger, José M. Estrelasingle cell gel electrophoresis assay for simple evaluation of cellular DNA damage. We used the assay kit from Abcam (ab238544) (Cambridge, UK) according to the manufacturer's instructions. Nicotinamide phosphoribosyltransferase

(13) c-MYC-Induced AP4 Attenuates DREAM-Mediated Repression by p53Cancers (Basel)February 11, 2023Markus Kaller, Wenjing Shi, Heiko HermekingTable S3. 2.7. Comet Assay Comet assays were performed using the Comet Assay Kit (3-well slides, ab238544, Abcam, Boston, MA, USA), as described previously [24], and imaged by using a microscope (Axiovert 25,

(14) Metabolic reprogramming of hepatocytes by Schistosoma mansoni eggsJHEP ReportsFebruary 1, 2023Verena von Bülow, Sarah Gindner, Anne Baier, Laura Hehr, Nicola Buss, Lena Russ, Sarah Wrobel, Victoria Wirth, Kuscha Tabatabai, Thomas Quack, et al.number of eggs was calculated per mg of liver tissue. Comet assay The alkaline comet assay (Abcam #ab238544) for assessing DNA damage in HepG2 cells was performed according to the manufacturer's protocol. Statistical

(15) Driving axon regeneration by orchestrating neuronal and non-neuronal innate immune responses via the IFNγ-cGAS-STING axisNeuronJanuary 18, 2023Wang X, Yang C, Wang X, Miao J, Chen W, Zhou Y, Xu Y, An Y, Cheng A, Ye W, et al.SuperScript II Reverse Transcriptase,Invitrogen,Cat#18064014 LightCycler 480 SYBR Green I Master,Roche,Cat#04707516001 Comet assay kit,Abcam,Cat#ab238544 RNAeasy mini Kit,QIAGEN,Cat#74104 ,, Experimental models: Cell lines,, ,, SpCas9 Neuro2a,GeneCopoeia,Cat#SL509 ,, Experimental models

(16) Nanoengineered Gallium Ion Incorporated Formulation for Safe and Efficient Reversal of PARP Inhibition and Platinum Resistance in Ovarian CancerResearch (Washington, D.C.)January 1, 2023Li Y, Cen Y, Tu M, Xiang Z, Tang S, Lu W, Zhang H, Xu J.independent experiments. Comet assay Comet assay was conducted using a Comet Assay Kit (Abcam, catalog no. ab238544). Briefly, cells treated as indicated were harvested and resuspended at a density of 1 × 105 cells/ml

(17) AP4 suppresses DNA damage, chromosomal instability and senescence via inducing MDC1/Mediator of DNA damage Checkpoint 1 and repressing MIR22HG/miR-22-3pMolecular CancerDecember 1, 2022Jinjiang Chou, Markus Kaller, Stephanie Jaeckel, Matjaz Rokavec, Heiko HermekingComet assay The comet assay was conducted according to instructions provided in Comet Assay Kit (3-well slides, ab238544, Abcam, USA). After detachment by trypsin cells were suspended in ice-cold PBS at 1 × 105 cells/ml.

(18) Chidamide and venetoclax synergistically exert cytotoxicity on multiple myeloma by upregulating BIM expressionClinical EpigeneticsDecember 1, 2022Liqin Cao et al.data. Alkaline comet assay Individual cellular DNA damage was detected by the alkaline comet assay (Abcam, ab238544, UK). At least 75 cells were analyzed per sample. The tail moment and the percentage of DNA in the comet

(19) Identification of a Novel Curcumin Derivative Influencing Notch Pathway and DNA Damage as a Potential Therapeutic Agent in T-ALLCancers (Basel)November 24, 2022Nadezda Zhdanovskaya, Sara Lazzari, Diego Caprioglio, Mariarosaria Firrincieli, Chiara Maioli, Eleonora Pace, Daniela Imperio, Claudio Talora, Diana Bellavia, Saula Checquolo, et al.were rinsed with water, fixed for 5 min in 70% ethanol and stained with Vista Green DNA Dye 10000X (ab238544; Abcam) for 15 min at RT in the dark, according to the manufacturer’s recommendations. Images were acquired

(20) Depletion of Fumarate Hydratase, an Essential TCA Cycle Enzyme, Drives Proliferation in a Two-Step ModelCancers (Basel)November 9, 2022Balakrishnan Solaimuthu, Michal Lichtenstein, Arata Hayashi, Anees Khatib, Inbar Plaschkes, Yuval Nevo, Mayur Tanna, Ophry Pines, Yoav D. ShaulMulti-Mode Reader. 2.9.2. Comet Assay The assay was conducted according to the Abcam comet assay kit (#ab238544) protocol. Briefly, 35 μL of suspended cells (1 × 106/mL) was mixed with 150 μL agarose, and 35 µL of

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Comet Assay Kit (3-well slides) from Abcam

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Comet Assay Kit (3-well slides) from Abcam

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